Victory would have sealed a place in the next round of the competition for the young Boro side, who raced into a three-goal lead through Hayden Hackney, Frankie Whelan and Alberto Balde. 

But the Royals hit back with four goals to deny last year's finalists progression to the next round of the Premier League Cup. 

Elsewhere, our U23s saw West Brom move above them in the PL2 league table after a 4-1 defeat away to the Baggies. 

Chris Willock opened the scoring for West Brom, with Rumarn Burrell equalising just before the break. 

However, the Baggies struck three times without reply in the second half to leapfrog Boro in the standings. 

Watch highlights from both games in the video player above.