It is the start of a busy week for Paul Jenkins' squad who face two cup ties in four days.
On Thursday they travel to Whitby for an NRSC Cup semi-final, but first is a trip to Wycombe Wanderers for a last 16 tie in the Premier League Cup.
They go there on the back of their biggest league win of the season, 4-1 at home to Blackburn Rovers.
"I was delighted with the performance that day, as much as the result," says Jenkins, the lead coach of this age group.
"We won't have the same team available, there's a suspension and international call-ups, but what we saw on Monday is the way I would like to think we will play again.
"We are at this stage of the competition on merit and we're out to give a good account of ourselves against a team who are not finding life easy in a higher division."
Boro's campaign so far has brought defeat home and away to Fulham and away to Charlton as well as wins at home to Charlton and away and at home to Wimbledon, a campaign that saw them finish second in their group and qualify for the knock-out stages.
Reading won their group, losing just one of their six games, finishing five points clear. They have lost their last two league games 4-0 and 5-1, but won 2-0 away to Manchester United in their game before that - their only win in their last six.
Kick-off is 1pm and the game will be decided on the day.